Hemoglobin

August 12, 2017 17:50 | Assays

Hb - the main component of red blood cells, is a complex protein consisting of heme and globin.The main function of Hb is to transfer oxygen from the lungs to the tissues, as well as in the removal of carbon dioxide from the body, and the regulation of CBS.Reference values ​​Hb concentrations in blood are shown in Table.[Tietz N., 1997].

Determination of Hb concentration in the blood plays an important role in the diagnosis of anemia.Conclusion The presence of anemia based on results of determination of Hb concentration and the Ht value of blood for men - Hb concentrations decrease below 140 g / l and Ht index less than 42%;Women - at least 120 g / l and 37% respectively.When anemia Hb concentration varies widely and depends on its shape, and severity.When iron deficiency anemia in patients with most of Hb decline relatively moderate (up to 85-114 g / l), at least more pronounced (up to 60-84 g / l).A significant reduction in the concentration of blood Hb (up to 50-85 g / l) is characteristic of acute b

lood loss, gipoplastiches Coy anemia, hemolytic anemia following hemolytic crisis, vitamin B12 deficiency anemia.The concentration of Hb 30-40 g / l - severe anemia rate at which the necessary urgent measures.The minimum concentration of Hb in the blood that is compatible with life - 10 g / l.

Hb concentration in the blood can be increased (up to 180-220 g / l and above) with myeloproliferative disorders (erythremia) and symptomatic erythrocytosis accompanying various states.Changes in Hb concentrations in various diseases are shown in Table .. Research Hb concentration dynamics provides important information about the clinical course of disease and the effectiveness of treatment.Falsely elevated Hb concentration in the blood is observed at gipertriglitseri-emy, leukocytosis above 25,0h109 / l, progressive liver disease, the presence of hemoglobin C or S, or multiple myeloma disease Valdenstrёma (the presence of easily precipitating globulin).

Table Diseases and conditions, accompanied by a change in the concentration of Hb in the blood

Table Diseases and conditions, accompanied by a change in Hb concentration in the blood

there are several types of Hb in human blood: HbA1 (96-98%), HbA2 (2-3%), HbF (1-2%), differing in amino acid composition globin, physical properties and affinity for oxygen.In infants prevails HbF - 60-80%, to 4-5-th month of life the amount is reduced to 10%.HbA appears in a 12-week fetus, in the adult it is the bulk of Hb.HbF in concentrations up to 10% can be detected with aplastic, megaloblastic anemia, leukemia;for large p-thalassemia, it may be 60-100% of the total Hb, with a small - 2-5%.Increased HbA2 fraction characteristic of p-thalassemia (with a large p-thalassemia - 4-10%, while small - 4-8%).

appearance of pathological forms due to a violation of the synthesis of Hb globin chains (hemoglobinopathies).The most common hemoglobinopathy S - sickle cell anemia.The main method of detecting pathological form of Hb - electrophoresis method.